Single molecule localization microscopy (SMLM) has enabled remarkable findings in microbiology. However, a Gaussian-shaped laser beam produces small and non-uniform illumination, which limits the ability to perform robust quantitative and statistical analyses of subcellular localizations from multiple cells. Abbelight’s patented ASTER technology overcomes this challenge by creating a uniform and large field of illumination, which facilitates SMLM imaging and analysis of hundreds of bacterial cells in a single acquisition with fine-scale localization. Here, we demonstrate the power of ASTER for high-throughput quantitative nanoscopy imaging, with the ability to achieve statistical significance in SMLM for both PAINT and PALM techniques.
