Total Internal Reflection Fluorescence (TIRF) microscopy is a powerful imaging technique widely used for studying molecular interactions and dynamics at the surface of cells. However, one of the main challenges in TIRF microscopy is dealing with samples that have varying refractive indices (RI), which can alter the TIRF imaging quality. Abbelight’s TIRF excitation technique addresses this issue by using automated calibration to define the experimental and precise TIRF angle, ensuring high accuracy and reproducibility for samples with different RI, hence making the process more reliable and adaptable to diverse experimental conditions.
